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e1 enzyme  (R&D Systems)


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    Structured Review

    R&D Systems e1 enzyme
    E1 Enzyme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ube1+e1/Recombinant+Human+GST-Ubiquitin+E1+Enzyme+(UBE1)+Protein%2C+CF/pm42784256-274-8-12
    Average 94 stars, based on 19 article reviews
    e1 enzyme - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Crystal structure of the SALL4-pomalidomide-cereblon-DDB1 complex.
    Article Snippet: Celgene Corporation, San Diego, CA, USA.. ✉e-mail: mmatyskiela@celgene.com; pchamberlain@celgene.com Thalidomide-dependent degradation of the embryonic transcription factor SALL4 by the CRL4CRBN E3 ubiquitin ligase is a plausible major driver of thalidomide teratogenicity.. The structure of the second zinc finger of SALL4 in complex with pomalidomide, cereblon and DDB1 reveals the molecular details of recruitment.

    Article Title: Proteolysis Targeting Chimera Degraders of the METTL3–14 m 6 A-RNA Methyltransferase
    Article Snippet: Human full-length cereblon-DDB1 and 6xHis-CUL4A-6xHis-RBX1 were coexpressed using the baculovirus/Sf9 insect cell expression system and purified by nickel affinity chromatography on a 5 mL HisTrap HP column (Cytiva) followed by anion exchange chromatography on a 5 mL HiTrap Q HP column (Cytiva) and a final gel-filtration step on a HiLoad 16/600 Superdex 200 pg column. .. Purified recombinant human UBE1 E1 (E-305–025) was purchased from R&D Systems, UbcH5a E2 (23–029) from Merck and ubiquitin (SBB-UP0013) from South Bay Bio. ..

    Article Title: Avadomide induces degradation of ZMYM2 fusion oncoproteins in hematologic malignancies
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2–616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Article Title: Methods for screening cereblon modifying compounds
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2-616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Article Title: Molecular glue CELMoD compounds are allosteric regulators of cereblon conformation
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2-616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Article Title: Methods for screening cereblon modifying compounds
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2-616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Recombinant:

    Article Title: Crystal structure of the SALL4-pomalidomide-cereblon-DDB1 complex.
    Article Snippet: Celgene Corporation, San Diego, CA, USA.. ✉e-mail: mmatyskiela@celgene.com; pchamberlain@celgene.com Thalidomide-dependent degradation of the embryonic transcription factor SALL4 by the CRL4CRBN E3 ubiquitin ligase is a plausible major driver of thalidomide teratogenicity.. The structure of the second zinc finger of SALL4 in complex with pomalidomide, cereblon and DDB1 reveals the molecular details of recruitment.

    Article Title: Proteolysis Targeting Chimera Degraders of the METTL3–14 m 6 A-RNA Methyltransferase
    Article Snippet: Human full-length cereblon-DDB1 and 6xHis-CUL4A-6xHis-RBX1 were coexpressed using the baculovirus/Sf9 insect cell expression system and purified by nickel affinity chromatography on a 5 mL HisTrap HP column (Cytiva) followed by anion exchange chromatography on a 5 mL HiTrap Q HP column (Cytiva) and a final gel-filtration step on a HiLoad 16/600 Superdex 200 pg column. .. Purified recombinant human UBE1 E1 (E-305–025) was purchased from R&D Systems, UbcH5a E2 (23–029) from Merck and ubiquitin (SBB-UP0013) from South Bay Bio. ..

    Article Title: Avadomide induces degradation of ZMYM2 fusion oncoproteins in hematologic malignancies
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2–616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Article Title: Methods for screening cereblon modifying compounds
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2-616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Article Title: Molecular glue CELMoD compounds are allosteric regulators of cereblon conformation
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2-616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Article Title: Methods for screening cereblon modifying compounds
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2-616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Ubiquitin Proteomics:

    Article Title: Crystal structure of the SALL4-pomalidomide-cereblon-DDB1 complex.
    Article Snippet: Celgene Corporation, San Diego, CA, USA.. ✉e-mail: mmatyskiela@celgene.com; pchamberlain@celgene.com Thalidomide-dependent degradation of the embryonic transcription factor SALL4 by the CRL4CRBN E3 ubiquitin ligase is a plausible major driver of thalidomide teratogenicity.. The structure of the second zinc finger of SALL4 in complex with pomalidomide, cereblon and DDB1 reveals the molecular details of recruitment.

    Article Title: Proteolysis Targeting Chimera Degraders of the METTL3–14 m 6 A-RNA Methyltransferase
    Article Snippet: Human full-length cereblon-DDB1 and 6xHis-CUL4A-6xHis-RBX1 were coexpressed using the baculovirus/Sf9 insect cell expression system and purified by nickel affinity chromatography on a 5 mL HisTrap HP column (Cytiva) followed by anion exchange chromatography on a 5 mL HiTrap Q HP column (Cytiva) and a final gel-filtration step on a HiLoad 16/600 Superdex 200 pg column. .. Purified recombinant human UBE1 E1 (E-305–025) was purchased from R&D Systems, UbcH5a E2 (23–029) from Merck and ubiquitin (SBB-UP0013) from South Bay Bio. ..

    Article Title: Avadomide induces degradation of ZMYM2 fusion oncoproteins in hematologic malignancies
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2–616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Article Title: Methods for screening cereblon modifying compounds
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2-616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Article Title: Molecular glue CELMoD compounds are allosteric regulators of cereblon conformation
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2-616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    Article Title: Methods for screening cereblon modifying compounds
    Article Snippet: .. Purified recombinant human Ube1 E1 (E-305), UbcH5a E2 (E2-616), and ubiquitin (U-100H) were purchased from R&D systems. ..

    other:

    Article Title: SALL4 mediates teratogenicity as a thalidomide-dependent cereblon substrate.
    Article Snippet: Celgene Corporation, San Diego, CA, USA.. *e-mail: pchamberlain@celgene.com Thalidomide was first marketed in the 1950s, when it found use as a mild sedative and in the treatment of morning sickness, among other indications1,2.. This led to a global epidemic of severe birth defects until it was established that thalidomide is a potent teratogen, with the most notable birth defect in surviving infants being phocomelia characterized by shortened or absent limbs3–6.



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